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Retinal fluorescence lifetime characteristics in patients with retinitis pigmentosa

Session Details

Session Title: Imaging IV

Session Date/Time: Sunday 20/09/2015 | 11:00-13:00

Paper Time: 11:56

Venue: Thalie.

First Author: : C.Dysli SWITZERLAND

Co Author(s): :    S. Wolf   M. Zinkernagel           

Abstract Details

PURPOSE:To characterize and quantify retinal autofluorescence lifetimes in patients with retinitis pigmentosa (RP) using a Fluorescence Lifetime Imaging Ophthalmoscopy (FLIO). FLIO is a novel imaging technique which allows for detection of structural and potentially also metabolic changes within the retina.

Setting:

This study was conducted at the department of ophthalmology at the University Hospital of Bern, Switzerland.

Methods:

A Fluorescence Lifetime Imaging Ophthalmoscope (FLIO, Heidelberg Engineering, Germany) was used for measurement of retinal autofluorescence decay times. Fluorescence was excited with a 473 nm laser and decay times were recorded in a short and in a long spectral channel (SSC: 498–560 nm, LSC: 560–720 nm). Corresponding optical coherence tomography (OCT) images were recorded. Totally 30 patients (age 45 ± 2.3, mean ± SEM) with retinitis pigmentosa were examined and mean lifetime values were compared with corresponding areas in age matched healthy control eyes.

Results:

Fluorescence lifetime values in the macular center of patients with retinitis pigmentosa were prolonged by 42% in the short and 28% in the long spectral channel compared to healthy control eyes (SSC: p=0.0026; LSC: p=0.0016). Peripheral areas with retinal atrophy were characterized by massively prolonged lifetime values.

Conclusions:

In vivo fluorescence lifetime measurement delivers disease specific lifetime patterns which might be used as a diagnostic tool and for monitoring of disease progression. FLIO potentially provides information about the functionality of individual retinal areas and layers.

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