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The role of retinal PAS-positive substances in development of dithizone-induced diabetic retinopathy

Session Details

Session Title: Vascular Diseases and Diabetic Retinopathy III

Session Date/Time: Saturday 19/09/2015 | 16:30-18:00

Paper Time: 17:34

Venue: Clio

First Author: : O.Dorokhova UKRAINE

Co Author(s): :    E. Maltsev   O. Zborovska           

Abstract Details

PURPOSE:Purpose: To compare retinal PAS-positive substances and neurodegenerative lesions in diabetic rabbits mice and rats. To study the possible nature and mechanisms of retinal neurodegeneration in dithizone-induced diabetes mellitus (DM) in rabbit.

Setting:

SI 'The Filatov Institute of Eye Diseases and Tissue Therapy of the National Academy of Medical Sciences of Ukraine'

Methods:

We modelled DM in Chinchilla rabbits by dithizone, in Wistar rats and CBA/C57B1xK/F1 mice by streptozotocine. The observation period did not exceed 6 months. Histological sections of enucleated animals eyes were stained with hematoxylin-eosin or by McManus-Hotchkiss and then studied by histochemical and histological methods.

Results:

We didn’t reveal (by using ordinary histological staining (hematoxylin-eosin)) any significant retinal changes in mice and rats by observing the animals from 2 to 6 months. There weren’t changes in stratified arrangement of nerve cells and amount of nerve cells. Nether signs of neurodegeneration no changes in PAS-positive substances were observed. The topography of the PAS-positive substances was also quite stable. We determined (by using ordinary histological staining (hematoxylin-eosin)) destruction of the photoreceptor layer, diminishing of neurons quantity in nuclear layers, diminishing of neurons in ganglion cell layer, thinning of the reticular layers, proliferation of glial elements, destruction of the pigment epithelium and focal retinal atrophic changes in retinas of diabetic rabbits after 16-17 weeks of observation. All of these described retinal damages were focal and combined with the obvious changes in the intensity and the type of PAS- positive substances in its layers.

Conclusions:

Dithizone-indued DM in rabbits is accompanied by pronounced retinal neurodegenerative signs. Severe retinal neurodegeneration is also combined with histochemical lesion of localization and amount of PAS positive substances in rabbits. Lesion of PAS positive substances may be presumable cause of diabetic neurodegeneration in retina.

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