Session Title: Vascular Diseases and Diabetic Retinopathy
Session Date/Time: Friday 18/09/2015 | 11:00-12:30
Paper Time: 12:04
First Author: : T.Pilkevych UKRAINE
Co Author(s): : V. Naumenko V. Vit
PURPOSE:The purpose of the study is detecting a correlation between structural changes in the retina, erythropoietin concentration in the peripheral blood and the vitreous and level of hemoglobin and red blood cells in experimental streptozotocin -induced diabetic rats in a follow-up period of 3 and 6 months.
SI 'The Filatov Institute of Eye Diseases and Tissue Therapy of the National Academy of Medical Sciences of Ukraine'
The experiment was performed in 60 Wistar rats. The rats were induced experimental diabetes by a single intraperitoneal injection of streptozotocin (SIGMA, the USA) in a dose of 65 mg per 1 kg body weight. All rats were divided into three groups: 10 rats (20 eyes) each. Group 1 – intact group, Group 2 – animals with modeled diabetes, and Group 3 - animals with modeled diabetes receiving recombinant erythropoietin (rEpo). rEpo administration started on the 10th day in a dose of 6 units per 1 kg of body weight three times a week during a month.
In 3 months the level of erythropoietin in peripheral blood of streptozotocin-induced diabetic rats was significantly increased comparing to intact rats. In 6 months there was no significantly increase in the level of erythropoietin in peripheral blood of streptozotocin-induced diabetic rats comparing to intact rats. As for streptozotocin-induced diabetic rats receiving rEpo, a significant increase in Epo concentration was noted in 3 and 6 months comparing to both Group 1 and 2. Erythropoietin concentration in the vitreous of streptozotocin-induced diabetic rats was significantly increased in 3 and 6 months comparing to intact animals. In streptozotocin-induced diabetic rats receiving rEpo there was a significant increase in Epo concentration in the vitreous in 3 and 6 months comparing to both intact and streptozotocin-induced diabetic rats. At 3 and 6 months after streptozotocin-induced diabetes onset all diabetic animals had severe structural changes in the retina, its histoarchitectonics was destroyed significantly.
Administration of recombinant erythropoietin reduced the odds of degenerative changes in the structural elements of the retina (neurons and glia), and in the vascular system of the retina, as well as degree of their development for 6 months.