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Retinal fluorescence lifetime characteristics in patients with fundus flavimaculatus

Session Details

Session Title: Imaging I

Session Date/Time: Friday 18/09/2015 | 08:00-09:30

Paper Time: 08:32

Venue: Athena

First Author: : C.Dysli SWITZERLAND

Co Author(s): :    S. Wolf   M. Zinkernagel           

Abstract Details

PURPOSE:To characterize and quantify retinal autofluorescence lifetimes in patients with fundus flavimaculatus using a Fluorescence Lifetime Imaging Ophthalmoscopy (FLIO). FLIO is a novel imaging technique which allows for detection of structural and potentially also metabolic changes within the retina.


This study was conducted at the department of ophthalmology at the University Hospital of Bern, Switzerland.


A Fluorescence Lifetime Imaging Ophthalmoscope (FLIO, Heidelberg Engineering, Germany) was used for measurement of retinal autofluorescence decay times. Fluorescence was excited with a 473 nm laser and decay times were recorded in a short and in a long spectral channel (SSC: 498–560 nm, LSC: 560–720 nm). Corresponding optical coherence tomography (OCT) images were recorded. Totally 13 patients (age 43.4± 2.6, mean ± SEM) with fundus flavimaculatus were examined and mean lifetime values were compared with corresponding areas in age matched healthy control eyes.


Fluorescence lifetime values in the unaffected retina were comparable with healthy control eyes (SSC: 250 ps, p=0.4; LSC: 300 ps, p=0.18). Hyperfluorescent lesions in general showed highly significantly prolonged fluorescence decay times (SSC and LSC: 450 ps). In central retinal atrophic areas, both, shortened and prolonged lifetimes, have been identified.


Analysis of retinal fluorescence lifetimes can be used for further characterization and differentiation of hyperfluoresecent lesions as well as hypofluorescent atrophic areas in fundus flavimaculatus. FLIO might emerge as a useful tool for imaging of retinal changes and monitoring of disease development over time.

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