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Retinal fluorescence lifetime characteristics in patients with fundus flavimaculatus

Session Details

Session Title: Imaging I

Session Date/Time: Friday 18/09/2015 | 08:00-09:30

Paper Time: 08:32

Venue: Athena

First Author: : C.Dysli SWITZERLAND

Co Author(s): :    S. Wolf   M. Zinkernagel           

Abstract Details

PURPOSE:To characterize and quantify retinal autofluorescence lifetimes in patients with fundus flavimaculatus using a Fluorescence Lifetime Imaging Ophthalmoscopy (FLIO). FLIO is a novel imaging technique which allows for detection of structural and potentially also metabolic changes within the retina.

Setting:

This study was conducted at the department of ophthalmology at the University Hospital of Bern, Switzerland.

Methods:

A Fluorescence Lifetime Imaging Ophthalmoscope (FLIO, Heidelberg Engineering, Germany) was used for measurement of retinal autofluorescence decay times. Fluorescence was excited with a 473 nm laser and decay times were recorded in a short and in a long spectral channel (SSC: 498–560 nm, LSC: 560–720 nm). Corresponding optical coherence tomography (OCT) images were recorded. Totally 13 patients (age 43.4± 2.6, mean ± SEM) with fundus flavimaculatus were examined and mean lifetime values were compared with corresponding areas in age matched healthy control eyes.

Results:

Fluorescence lifetime values in the unaffected retina were comparable with healthy control eyes (SSC: 250 ps, p=0.4; LSC: 300 ps, p=0.18). Hyperfluorescent lesions in general showed highly significantly prolonged fluorescence decay times (SSC and LSC: 450 ps). In central retinal atrophic areas, both, shortened and prolonged lifetimes, have been identified.

Conclusions:

Analysis of retinal fluorescence lifetimes can be used for further characterization and differentiation of hyperfluoresecent lesions as well as hypofluorescent atrophic areas in fundus flavimaculatus. FLIO might emerge as a useful tool for imaging of retinal changes and monitoring of disease development over time.

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