First Author: J.Kim SOUTH KOREA
Co Author(s): C.S. Kim Y.M. Lee J. Kim 0 0 0 0 0 0 0 0 0
Back to previous
Many dietary constituents may be beneficial for the prevention and treatment of diabetes. There is little evidence that food factors themselves can be directly beneficial for preventing diabetes-induced retinal vascular injury. The aim of this study was to determine whether dietary grape seed supplement help to prevent diabetes-induced retinal vascular dysfunction in vivo.
Grape seed extracts (GSE, 100mg/kg) were orally administration in db/db mice for twelve weeks.
Blood-retinal barrier (BRB) permeability was quantified using fluorescein angiography. The distribution of tight junction protein was analyzed by whole mount immunofluorescence staining. Advanced glycation end products (AGEs) accumulation, oxidative DNA damage and pericyte apoptosis were investigated in retinal vessels.
At 20 weeks of age, all db/db mice exhibited hyperglycaemia, and GSE did not affect the blood glucose levles and body weight during the experiments. The treatment of GSE decreased significantly vascular AGEs accumulations and oxidative DNA damage (p<0.01 vs vehicle). In dextran-FITC angiography, the fluorescein leakage was significantly reduced in db/db mice treated with GSE. Retinal pericyte apoptosis was markedly inhibited by GSE. GSE treatment also decreased retinal VEGF expression and the degradation of occludin, an important tight junction protein in db/db mice.
In db/db mice, this well-known dietary supplement prevents early diabetic retinopathy. These findings have important implications for prevention of diabetic retinopathy using dietary grape seed supplements.