First Author: K.Georgakopoulos GREECE
Co Author(s): O. Makri P. Plotas N. Farmakakis A. Exarchou 0 0 0 0 0 0 0 0 0
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To evaluate the efficacy of ofloxacin 0.3 % in eradication of conjunctival bacterial flora in diabetic patients undergoing intravitreal injections.
Department of Ophthalmology, University Hospital of Patras
Diabetic patients scheduled to undergo intravitreal injection of ranibizumab due to diabetic macular edema were enrolled in the study. In Group 1 patients received ofloxacin eye drops for 1 day before intravitreal injection (1 drop 4 times the day before intravitreal injection); in Group 2 ofloxacin was administered for 1 hour before intravitreal injection (every 15 minutes for a total of 4 applications) while Group 3 ofloxacin was administered for 1 day before and for 1 hour at the day of injection (overall 8 doses). Samples were collected from the injection site area at baseline and after antibiotic administration before the intravitreal injection. The positive or negative culture results found in BACTEC broth and the positive cultures in blood agar and Sabouraud’s dextrose agar plates were measured.
In Group 1 BACTEC broth positive cultures decreased from 84.4% at baseline to 50% after ofloxacin administration (p=0.007). In the same group, blood agar positive cultures reduced from 65.63% at baseline to 34.38% (p = 0.02). In Group 2 the number of positive cultures was also significantly decreased (from 79.3% at baseline to 48.28 %; p=0.027) in BACTEC broth and from 68.97% to 37.13% (p=0.034) in blood agar. Finally, in Group 3 positive cultures decreased from 77.42% at baseline to 32.26% (p=0.0008) and from 58.06% at baseline to 22.58% (p=0.009) in BACTEC broth and blood agar respectively. No microorganisms were isolated from the Sabouraud’s dextrose agar plates.
The reduction of bacteria from the conjunctiva that is caused after the combined 1day/1 hour (8 doses) ofloxacin administration in diabetic patients is extremely effective. The application of topical ofloxacin for 1 day (4 doses) or 1 hour (4 doses) before the injection also reduces significantly the bacterial conjunctival flora.