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Time Dependent Changes of Cellular Components Proliferation after Laser Photocoagulation in Mouse Chorioretinal Tissue

Poster Details

First Author: T.K.Park SOUTH KOREA

Co Author(s):    S.H. Lee   Y.H. Ohn               0   0 0   0 0   0 0   0 0

Abstract Details


To investigates the time course of proliferation of cellular components and identify the types of proliferating cells after laser photocoagulation in mouse chorioretinal tissue


Experimental study


Forty eight C57BL/6J mice were subjected to bilateral laser photocoagulation. Mice were divided into two groups, 24 mice each, and intraperitoneal bromodeoxyuridine (BrdU) injection was performed to each group with different injection pattern, continuous injection (group 1) and periodical injection (group 2). Each group was again divided into 4 subgroups based on BrdU injection period (1a to 1d, and 2a to 2d, n=6 each). Subgroups from each group received IP BrdU injection during following intervals: group 1a- 0-3 days, group 1b- 0-7 days, group 1c- 0-14 days, and group 1d- 0-28 days after laser photocoagulation; and group 2a- 0-3 days, group 2b- 4-7 days, group 2c- 8-14 days, and group 2d- 15-28 days after laser photocoagulation. The eyes were enucleated at the completion of each injection intervals for both groups, and were examined by immunohistochemistry for quantitative analysis and identification of proliferating cell types


The sections from the center of laser burn demonstrated a burst of BrdU labeling around the laser site, in both supra-RPE and sub-RPE area. Group 1 showed a burst of BrdU labeling in the choroid, and the sclera, with a few labeling in the subretinal space and the RPE layer in the first 3 days after laser photocoagulation. After 7 days of laser treatment, the supra-RPE layer showed a slight decrease in the number of BrdU-positive cells, while the sub-RPE layer demonstrated a gradual increase in the number of BrdU-positive cells until 28 days of laser treatment. On the other hand, group 2 demonstrated peak increase in the number of proliferating cells during 0-3 days interval in both supra-RPE and sub-RPE layer, and the number of labeling decreased thereafter. In immunohistochemisty, these proliferating cells were positive not only for RPE65 but also for CD11b, F4/80, GFAP, and beta-catenin. Among these various cell markers, CD11b positive cells were observed the most at all time points


This study demonstrated the temporal patterns of proliferation of various cellular components in mouse chorioretinal tissue after laser photocoagulation, and the results of this study may be useful in further evaluations of the biological processes involved in the morphological changes of the retina occurring after laser photocoagulation

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