First Author: T.Bertelmann GERMANY
Co Author(s): T. Stief S. Schulze W. Sekundo 0 0 0 0 0 0 0 0 0
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To investigate whether visual opsins can be detected in human vitreous liquid.
Pilot study including 22 vitreal samples which were harvested at the beginning of a standard 23-gauge three-port pars plana vitrectomy for macular pucker, diabetic vitreous hemorrhage or vitreal floater removal as well as macular hole closure or vitreomacular traction relief from the central vitreous body.
All samples were immediately stabilized by human albumin (2.5% final conc.) and arginine (1.25M final conc.) and subsequently frozen. Short-wavelength cone opsin concentrations were analyzed by enzyme immune essay (EIA) with anti-proteolytic 400mM arginine, pH 8.7, in the antigen capture phase.
Intravitreal short-wavelength cone opsins were detected in all analyzed samples and respective concentrations were 157pg/ml ± 73pg/ml (range: 27pg/ml - 286pg/ml). No adverse events or serious side effects occurred.
Cone opsins are present in human vitreous fluid. Further studies will quantify the intravitreal opsin pattern of all visual opsins and compare these concentrations between different vitreoretinal diseases. This in turn might offer a better pathophysiological understanding and new diagnostic and therapeutic strategies for various eye pathologies. Soluble opsins could be a biomarker for retinal damage comparable to creatinine for kidney damage or troponin I for ischemic heart damage.