First Author: G.Levytska UKRAINE
Co Author(s): 0 0 0 0 0 0 0 0 0
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To compare the concentration of amino acids in vitreous fluid in patients with rhegmatogenous retinal detachment (RRD) to that of control postvitrectomy fluid of vitreous cavity in early postoperative period.
Known that glutamate, nerve degeneration mediator in the injured central nervous system, plays an important role in retinal apoptosis.
This prospective, observational study measured amino-acid levels (n-15) in vitreous fluid (n-35) of patients undergoing pars plana vitrectomy for RRD with inferior breaks and in postvitrectomy vitreous fluid (n-67). All patients (n-88) with macula-off RRD had inferior breaks and underwent vitrectomy, fluid-gas exchange through own retinal tear, laser coagulation and gas (C3F8) tamponade. There were no retinothomy, heavy liquids or silicone oil. According to our method, in RRD cases with inferior breaks additional gas tamponade was performed to correct volume of gas bubble after vitrectomy. Postvitrectomy fluid we obtained during additional gas-fluid exchange. Fluids were analyzed using high-pressure liquid chromatography.
Mean levels of glutamine acid (2,174±2,553 ng/ml), glycine (0,822±0,655), aspartate (1,647±1,358), alanine (1,583±1,438), tyrosine (0,248±0,216), valine (0,827±0,774), isoleucine (0,248±0,208), leucine (1,474±1,307), histidine (0,251±0,263), and phenylalanine (0,490±0,420) in postvitrectomy fluid at 1-2 days after surgery were significantly elevated comparing to control of initial vitreous fluid of patients with primary rhegmatogenous retinal detachment: glutamine acid (0,344±0,182 ng/ml), glycine (0,078±0,046), aspartate (0,134±0,078), alanine (0,177±0,093), tyrosine (0,046±0,021), valine (0,083±0,046), isoleucine (0,046±0,034), leucine (0,140±0,131), histidine (0,058±0,0038), and phenylalanine (0,064±0,039) according (all P˂0,001).
This study found significantly increased level of amino acids in postvitrectomy fluid in early postoperative period compared to its initial levels in vitreous. Additional studies are needed to investigate any possible association between amino acids levels and visual acuity, and to find drugs as a possible therapeutic modality.