First Author: M.Santiago SPAIN
Co Author(s): M. Bande Rodriguez M.J. Blanco-Teijeiro P. Mera C. Capeans M. Pardo Perez A. Pineiro Ces 0 0 0 0 0 0 0 0 0
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DJ-1/PARK7 is an oncoprotein whose aberrant expression has been closely related to the proliferation, metastasis, occurrence, and prognosis of the malignant tumors by down-regulating the phosphatase and tensin homologue (PTEN). The overexpression of DJ-1 has been previously described by our group in uveal melanoma (UM) tumors. Additionally, we detected DJ-1 in UM tumor secretomes and in UM patients sera. Importantly, we have described that high DJ-1 circulating levels are associated with choroidal nevi transformation risk factors.
To investigate the effect of DJ-1 inhibition on the proliferation of uveal melanoma established cells lines because the pathophysiological role of DJ-1 in UM is still poorly understood
Small interfering RNA (siRNA) directed against DJ-1 (Human PARK7siRNAON-TARGETplus SMARTpool, Thermo Scientific Dharmacon) or control (ON-targetplus non-targeting siRNA, Thermo Scientific Dharmacon) was introduced in UM-A, SP6.5, and 92.1 cell lines with Lipofentamine. Dynamic cell proliferation of silenced DJ-1 vs. non-silenced cells was assayed by real-time monitoring using xCelligence System (Roche®).
Down-regulation of DJ-1expression in UM cells showed a significant decrease of cell proliferation compared to those transfected with control siRNA.
The expression of DJ-1 oncogene may play a role in the proliferation of uveal melanoma cells in vitro.