london euretina

This meeting has been awarded 20 CME credits

Security Notice

Please note that Kuoni are our only destination management company. Other venders indicating that are operating for the society should be ignored. We never use western union as a payment portal


Silencing of PARK7/DJ-1 gene by siRNA inhibits uveal melanoma cell proliferation in vitro

Poster Details

First Author: M.Santiago SPAIN

Co Author(s):    M. Bande Rodriguez   M.J. Blanco-Teijeiro   P. Mera   C. Capeans   M. Pardo Perez   A. Pineiro Ces   0   0 0   0 0   0 0   0 0

Abstract Details


DJ-1/PARK7 is an oncoprotein whose aberrant expression has been closely related to the proliferation, metastasis, occurrence, and prognosis of the malignant tumors by down-regulating the phosphatase and tensin homologue (PTEN). The overexpression of DJ-1 has been previously described by our group in uveal melanoma (UM) tumors. Additionally, we detected DJ-1 in UM tumor secretomes and in UM patients sera. Importantly, we have described that high DJ-1 circulating levels are associated with choroidal nevi transformation risk factors.


To investigate the effect of DJ-1 inhibition on the proliferation of uveal melanoma established cells lines because the pathophysiological role of DJ-1 in UM is still poorly understood


Small interfering RNA (siRNA) directed against DJ-1 (Human PARK7siRNAON-TARGETplus SMARTpool, Thermo Scientific Dharmacon) or control (ON-targetplus non-targeting siRNA, Thermo Scientific Dharmacon) was introduced in UM-A, SP6.5, and 92.1 cell lines with Lipofentamine. Dynamic cell proliferation of silenced DJ-1 vs. non-silenced cells was assayed by real-time monitoring using xCelligence System (RocheĀ®).


Down-regulation of DJ-1expression in UM cells showed a significant decrease of cell proliferation compared to those transfected with control siRNA.


The expression of DJ-1 oncogene may play a role in the proliferation of uveal melanoma cells in vitro.

Back to previous
EURETINA, Temple House, Temple Road, Blackrock, Co Dublin. | Phone: 00353 1 2100092 | Fax: 00353 1 2091112 | Email:

Privacy policyHotel Terms and Conditions Cancellation policy