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Regenerative effect of range neurotrophic factors in high dose AGEs exposed isolated rat retinas.

Session Details

Session Title: Quick Fire Free Paper 1

Session Date/Time: Thursday 11/09/2014 | 08:00-10:00

Paper Time: 08:50

Venue: Boulevard D

First Author: : G.Bikbova JAPAN

Co Author(s): :    T. Oshitari   S. Yamamoto           

Abstract Details

Purpose:

To determine the effect of high dose advanced glycation end-products (AGE) on neurite regeneration in isolated rat retinas and the regenerative effect of different neurotrophic factors in AGE exposed retinas.

Setting:

Chiba University, Department of Ohthalmology and Visual Science

Methods:

Retinal explants of 4 adult SD rats were three-dimensionally cultured in collagen gel and were incubated either in; 1) serum free control culture media, 2) 100 μg/ml glucose-AGE-BSA; glycolaldehyde-AGE-BSA; glyceraldehyde-AGE-BSA media, 3 ) glucose-AGE, glycol - AGE, glycer - AGE+100ng/ml neurotrophin 4 (NT-4) media, 4) glucose-AGE, glycol - AGE, glycer - AGE+100ng/ml hepatocyte growth factor (HGF) media, 5) glucose-AGE, glycol - AGE, glycer - AGE+100ng/ml glial cell line derived neurotrophic factor (GDNF) media, 6) glucose-AGE, glycol - AGE, glycer - AGE+100ng/ml Tauroursodeoxycholic acid (TUDCA) media. After 7 days, the number of regenerating neurites from the explants was counted under a phase-contrast microscope. Statistical analysis was performed by one-way ANOVA.

Results:

In retinas incubated with AGEs (glucose-AGE, glycol-AGE, and glycer-AGE), the numbers of regenerating neurites were fewer than in retinas without AGE. (7,16±4,38 vs 15,6±5,58 P = 0,0046, 4,00±2,45 vs 15,6±5,58 P < 0,0001,4,69±4,70 vs 15,6±5,58 P = 0,0003). All neurotrophic factors increased the neuritis regeneration in AGEs exposed retinas, but the most significant regenerative effect was noticed in NT4 group. 60,66±27,99 vs 7,16±4,38, P < 0,0001 in NT4 group; 45,00±16,05 vs 7,16±4,38 , P < 0,0001, in HGF group; 23,75±5,56 vs 7,16±4,38 ,P = 0,0061 in GDNF group ; 39,6±9,07 vs 7,16±4,38, P < 0,0001 in TUDCA group supplemented to glucose –AGE incubated retinas.

Conclusions:

NT-4 significantly enhances neurite regeneration in retinas exposed to high dose of AGE comparing with other neurotrophic factors such as HGF, GDNF and TUDCA.

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