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Regenerative effect of range neurotrophic factors in high dose AGEs exposed isolated rat retinas.

Session Details

Session Title: Quick Fire Free Paper 1

Session Date/Time: Thursday 11/09/2014 | 08:00-10:00

Paper Time: 08:50

Venue: Boulevard D

First Author: : G.Bikbova JAPAN

Co Author(s): :    T. Oshitari   S. Yamamoto           

Abstract Details


To determine the effect of high dose advanced glycation end-products (AGE) on neurite regeneration in isolated rat retinas and the regenerative effect of different neurotrophic factors in AGE exposed retinas.


Chiba University, Department of Ohthalmology and Visual Science


Retinal explants of 4 adult SD rats were three-dimensionally cultured in collagen gel and were incubated either in; 1) serum free control culture media, 2) 100 μg/ml glucose-AGE-BSA; glycolaldehyde-AGE-BSA; glyceraldehyde-AGE-BSA media, 3 ) glucose-AGE, glycol - AGE, glycer - AGE+100ng/ml neurotrophin 4 (NT-4) media, 4) glucose-AGE, glycol - AGE, glycer - AGE+100ng/ml hepatocyte growth factor (HGF) media, 5) glucose-AGE, glycol - AGE, glycer - AGE+100ng/ml glial cell line derived neurotrophic factor (GDNF) media, 6) glucose-AGE, glycol - AGE, glycer - AGE+100ng/ml Tauroursodeoxycholic acid (TUDCA) media. After 7 days, the number of regenerating neurites from the explants was counted under a phase-contrast microscope. Statistical analysis was performed by one-way ANOVA.


In retinas incubated with AGEs (glucose-AGE, glycol-AGE, and glycer-AGE), the numbers of regenerating neurites were fewer than in retinas without AGE. (7,16±4,38 vs 15,6±5,58 P = 0,0046, 4,00±2,45 vs 15,6±5,58 P < 0,0001,4,69±4,70 vs 15,6±5,58 P = 0,0003). All neurotrophic factors increased the neuritis regeneration in AGEs exposed retinas, but the most significant regenerative effect was noticed in NT4 group. 60,66±27,99 vs 7,16±4,38, P < 0,0001 in NT4 group; 45,00±16,05 vs 7,16±4,38 , P < 0,0001, in HGF group; 23,75±5,56 vs 7,16±4,38 ,P = 0,0061 in GDNF group ; 39,6±9,07 vs 7,16±4,38, P < 0,0001 in TUDCA group supplemented to glucose –AGE incubated retinas.


NT-4 significantly enhances neurite regeneration in retinas exposed to high dose of AGE comparing with other neurotrophic factors such as HGF, GDNF and TUDCA.

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