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Cellular response to selective retina therapy by microsecond-pulsed laser equipped with automated real-time reflectometry

Session Details

Session Title: New Drug Treatment

Session Date/Time: Saturday 13/09/2014 | 14:30-16:30

Paper Time: 15:58

Venue: Boulevard F

First Author: : T.K.Park SOUTH KOREA

Co Author(s): :    S.H. Lee   H.D. Kim   R. Brinkmann        

Abstract Details

Purpose:

To evaluate the cellular response to the selective retina therapy (SRT) by microsecond-pulsed laser irradiation with automatic real-time reflectometry in mouse retinal tissue and compare it with continuous wave laser photocoagulation (cwPC)

Setting:

Experimental study

Methods:

Thirty C57BL/6J mice received 10 shots of SRT on the right eye and 10 shots of cwPC on the left eye around the optic disc in a concentric pattern. Animals were sacrificed at 12 hours, 24 hours, 3 days, and 7 days after SRT and cwPC. The eyes were then enucleated and immunohistochemistry was performed using GFAP, F4/80, CD11b, beta-catenin, and RPE65 to identify various cellular responses to SRT and cwPC at each different time intervals, and the results were compared between the two laser modalities

Results:

The sections from SRT lesions at 12 hours demonstrated increased irregularity of the RPE monolayer structure with lengthening of the outer segments of photoreceptors without morphological change in the inner segments and the external limiting membrane, and these changes recovered completely by 7 days of SRT. In immunohistochemistry, slightly increased GFAP expression was detected in the inner half of the retina at as early as 12 hours of SRT localized just above the lesions, which diminished after 7 days of treatment. On the other hand, cwPC treated retina demonstrated generalized GFAP expression beyond the laser lesions with time dependent increase until 7 days of laser photocoagulation. Additionally, SRT lesions showed just a few of F4/80 and CD11b positive cells in the inner retina and the choroid, but not in subretinal space, while cwPC lesions demonstrated many of F4/80 and CD11b positive cells mainly in the subretinal space and the choroid. Expression for beta-catenin and RPE65, however, did not increase significantly around the SRT lesion

Conclusions:

In this study, when compared to cwPC, SRT produced only a small amount of cellular responses, including gliosis and inflammatory reactions. The results of this study may be useful in further evaluations on the therapeutic effects of SRT in retinal disorders

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