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Plasma coenzyme Q10 levels in type 2 diabetic patients with diabetic retinopathy

Session Details

Session Title: Vascular Diseases III

Session Date/Time: Sunday 29/09/2013 | 08:00-10:00

Paper Time: 08:48

Venue: Hall 3 (Level 0)

First Author: O.Ates TURKEY

Co Author(s):    H. Bilen   S. Keles   H. Alp   M. Kele?     

Abstract Details

Purpose:

The aim of this study was to determine the relationship between proliferative diabetic retinopathy and plasma coenzyme Q10 concentration.

Setting:

Patients with type 2 diabetes and DRP were determined to be the case group (n=50). The control group was consist of healthy individuals (n=50) Plasma coenzyme Q10 and malondialdehyde (MDA) levels were measured in both groups.

Methods:

Analyses of ubiquinol-10 and ubiquinone-10 were performed according to Mosca et al. This method is performed by forcing the oxidation of CoQ10 in the plasma sample by treating it with para-benzoquinone fol-lowed by extraction with 1-propanol and direct injection into the HPLC apparatus. Preoxidation of the sample ensures quantification of total CoQ10 by UV detection.Analysis of plasma for MDA by HPLC MDA concentrations in the blood plasma sample were measured by HPLC with fluorescent detection (HPLC-FLD).

Results:

Ubiquinone-10 (Coenzyme Q10) levels in proliferative diabetic retinopathy and control subjects are 3.81±1.19 µmol/L and 1.91±0.62 µmol/L, respectively. Plasma MDA levels in proliferative diabetic retinopathy and control subjects were 8.16±2 µmol/L and 3.44±2.08 µmol/L, respectively. Ratio of Ubiquinol-10/ubiquinone-10 in proliferative diabetic retinopathy and control subjects were 0.26±0.16 and 1.41±0.68, respectively. Ratio of ubiquinol-10/ubiquinone-10 was significantly lower in patients with proliferative diabetic retinopathy than the control groups (p<0.05). There was a significant difference between proliferative diabetic retinopathy and control subjects for Plasma MDA levels (p<0.05). There was a significant negative correlation was between MDA levels and the ratio of ubiquinol-10/ ubiquinone-10.

Conclusions:

The ratio of ubiquinol-10/ubiquinone-10 was found lower in patients with proliferative diabetic retinopathy. High levels of plasma Ubiquinol-10/ubiquinone-10 ratio indicate the protective effect on diabetic retinopathy.

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