Session Title: Quick Fire Free Paper Session 04
Session Date/Time: Sunday 29/09/2013 | 11:00-13:00
Paper Time: 11:55
Venue: Hall C (Level 1)
First Author: Y.Li CHINA
Co Author(s): T. Sun L. Hao
To evaluate the association between single nucleotide polymorphisms (SNPs) of TLR2 and TLR4 gene and proliferative diabetic retinopathy (PDR) in Chinese patients with type 2 diabetes mellitus (T2DM).
Beijing Institute of Ophthalmology， Beijing Tongren Hospital , Capital Medical University, Beijing, China
A total of 217 type 2 diabetes patients were continuously enrolled in our study, and they were divided into PDR group and NPDR group according fluorescein angeography examination. Among them, 113 patients were in PDR group and 104 patients in NPDR group. Eight tag SNPs in the TLR4 gene (rs10983755，rs7037225，rs7856729，rs11536879，rs12377632，rs7037117rs，7873784，and rs11536889) and five tag SNPs in the TLR2 gene (rs3804100，rs7656411，rs11938228，rs75489577，and rs7696323) were genotyping using a high-throughput Sequenom genotyping platform (matrix－assisted laser desorption／Ionization time of flight mass spectrometry，MALDI－TOF－MS), and an association analysis was performed using PLINK software package.
After controlling for sex, HbA1c, and duration of disease, three tag SNPs in TLR4 gene were significantly associated with PDR in the cohort of this study. The AA genotype of rs 10983755 [P = 0.0019, OR 0.34 (0.172-0.6721)], the TT genotype of rs12377632 [P = 0.0038, OR 0.5072 (0.3204-0.803)], and the GG genotype of rs7037117[P = 0.0103, OR 0.4353(0.2307-0.8214)] were significantly associated. The A allele of rs 10983755 [P = 0.0006, OR 0.4164 (0.2572-0.6743) ] and the T allele of rs12377632 [P = 0.0046, OR 0.4043 (0.241-0.6783) ] were significantly associated. Haplotype analyses showed a significant association of PDR with the haplotype CAC, as defined by rs1098755，rs11536879 and rs12377632. None of the SNPs in TLR2 showed a significant association with PDR in our study.
Our data suggest that SNPs in TLR4 could reduced the risk of PDR in Chinese patients with T2DM.