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Study of proliferative vitreoretinal membrane using atomic force microscopy and scanning electron microscopy

Session Details

Session Title: Quick Fire Free Paper Session 03

Session Date/Time: Sunday 29/09/2013 | 08:30-09:30

Paper Time: 08:35

Venue: Hall C (Level 1)

First Author: A.Karamitsos GREECE

Co Author(s):    S. Logothetidis   M. Seitanidou   L. Lamprogiannis   N. Dragoumis     

Abstract Details

Purpose:

Analysis of topography, morphology and properties of the vitreo-retinal proliferative membrane.

Setting:

1. 1st Ophthalmology Clinic, AHEPA University Hospital, AUTH, Thessaloniki, Greece 2. Laboratory for Thin Films - Nanosystems & Nanometrology, Department of Physics, AUTH, Thessaloniki, Greece

Methods:

Surgically removed vitreo-retinal proliferative membranes from patients with retinal detachment and proliferative vitreo-retinopathy were analyzed for this study. All samples were obtained during a standard three port pars plana vitrectomy. Atomic force microscopy (AFM) of membranes was conducted. The membranes were also chemically treated, in order to be fixated and dehydrated and then to be analyzed with the scanning electron microscope (SEM).

Results:

Topographic analysis of the membranes suggested certain heterogeneity in their surface. Quantitative measurements such as root mean square roughness ranged from 41,86 nm to 179,42 nm and the membrane’s maximum thickness ranged from 270,69 nm to 1176,87 nm. AFM also provided information as to the properties of the samples. Globular structures were present on the surface of all samples. This surface morphology was also observed with the SEM.

Conclusions:

Quantitative analysis of roughness, morphology and properties of human proliferative vitreo-retina membrane could provide essential information toward the improvement of existing surgical instruments for more efficient and safer surgical removal of this pathologic membrane.

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