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Structured illumination ophthalmoscope for high-resolution fluorescence imaging of retinal pigment epithelium

Session Details

Session Title: Imaging I

Session Date/Time: Thursday 26/09/2013 | 08:30-10:30

Paper Time: 09:34

Venue: Hall 3 (Level 0)

First Author: S.Dithmar GERMANY

Co Author(s):    G. Best   S. Rossberger   T. Ach   S. Pollithy     

Abstract Details

Purpose:

Structured illumination microscopy (SIM) enables high resolution light microscopy of fluorescent molecules in a 110 nanometer range. In this study, SIM was applied for fluorescence imaging of the fundus.

Setting:

Department of Ophthalmology, University of Heidelberg

Methods:

We developed a device for high-resolution fluorescence imaging of the retinal pigment epithelium (RPE) in the intact eye using SIM. To study the setup's imaging capabilities, we used a model eye containing RPE-choroid specimen.

Results:

Lateral resolution of < 2μm and high contrast was achieved allowing us to resolve the fluorescence distribution in single cells.

Conclusions:

Applicability of structured illumination funduscopy was shown using an artificial eye. It was possible to exceed the resolution of state of the art ophthalmoscopes.

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