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Effects of blue light on chemokines expression in human retinal pigment epithelium cells

Session Details

Session Title: AMD I

Session Date/Time: Thursday 26/09/2013 | 08:30-10:30

Paper Time: 08:54

Venue: Hall 1 (Level 2)

First Author: S.Chen CHINA

Co Author(s):                  

Abstract Details


To observe the effects of blue light on human RPE cells replicative senescence and expression of chemokines in RPE cell. It is to be discussed the role of blue light, RPE replicative senescence and inflammatory reaction in pathogenesis of age-related macular degeneration (AMD)


Tianjin Eye Hospital


Healthy human eyeball was used for experiments. Primary human RPE culture was harvested with trypsin digestion. The cells were identified with immunohistochemistry. The blue light source of light emitting diode was used to cause replicative senescence and detect senescence associated beta-galactosidae(SA-β-Gal) activity. RT-PCR assay to detect expressions of chemokines IL-8、MCP-1 in RPE cells


This primary culture resulted in cells with well-preserved morphology using trypsin. The next passages were grew well.The number of the replicative senescence cells and the exprressions chemokines IL-8、MCP-1 in RPE cells with blue particles increased as the increase of exposure intensity. The difference within the different light exposure intensity had statistics significance (P<0.01)


The replicative senescence of human RPE cells are positive correlation with blue light . The blue light increases the expressions of chemokins IL-8、MCP-1 in human RPE cells

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