Session Title: Quick Fire Free Paper Session 01
Session Date/Time: Thursday 26/09/2013 | 08:30-10:30
Paper Time: 09:30
Venue: Hall G1 (Level 2)
First Author: L.Lytvynchuk UKRAINE
Co Author(s): A. Sergiienko G. Lavrenchuk V. Siriachenko
To study biological and physical properties of emulsified silicon oil in vitro and vivo.
Investigation of biological and physical properties of emulsified SO (SO) is essential to understand pathological mechanisms caused by prolonged endotamponades.
Twelve samples of emulsified silicon oil taken from anterior chamber tapping during silicon oil extraction after PPV due to RD were studied. Cultivation of silicon fragments with fibroblast-like cell strain L929 was performed to evaluate cellular effects. Ten samples were injected into the vitreous cavity of the rats eyes to evaluate cellular effects of emulsified SO in the condition of ocular hypertension. Two samples were studied under microscope in BSS drop to evaluate physical properties in dormant conditions.
Over 60% of SO droplets in all the samples were sized of 0.1 micron. Cultivation of emulsified SO with fibroblast-like cell strain an incomplete phagocytosis of SO droplets by cellular culture was observed. Slight inhibition of cellular mitosis and proliferative activity was observed as well. In the condition of ocular hypertension in rats eyes droplets of emulsified SO have migrated into the ocular tissues with prominent phagocytosis within different cellular type. Incomplete phagocytosis of SO droplets was observed within epithelial cells of trabecular meshwork of the rat’s eye. In dormant conditions emulsified SO droplets displayed chaotic movement which resembles Brownian movement.
Small size of emulsified SO droplets makes them invisible for any investigation technique. Chaotic movement of SO droplets can explain the interaction within ocular tissues and cellular phagocytosis.