First Author: T.Kiseleva RUSSIA
Co Author(s): V. Neroev A. Chudin A. Shchipanova I. Horoshilova-Maslova
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To study the effect of antioxidant resveratrol on ocular blood flow (OBF) of rats with retinal ischaemia-reperfusion injury.
The Helmholtz Moscow Research Institute of Eye Diseases
The experimental study was performed in 50 Wistar rats (50 eyes). In the first stage of the study all rats were divided into two Groups: Group I consisted of 20 intact rats (20 eyes), Group II - 30 rats (30 eyes) were orally administered resveratrol with 20 mg/kg for one month. In the second stage we performed modeling of one-sided ischaemia-reperfusion of the eye by increasing IOP. Group I animals were divided into the subGroup IA - 10 animals (10 eyes) undergoing ischaemia-reperfusion modeling of one eye and IB (control) consisted of 10 intact rats (10 eyes). All animals of the Group II kept receiving resveratrol and also were undergoing modeling ischaemia-reperfusion of the eye. OBF was estimated using power Doppler (PD) and pulsed-wave spectral Doppler ultrasonography before ischaemia-reperfusion and 3, 7, 30 days after. We estimated Doppler frequency spectrum in the following vessels: central retinal artery (СRA), long posterior ciliary arteries (LPCA), superior (SOA) and inferior ophthalmic arteries (IOA). The main quantitative characteristics for OBF assessment included peak-systolic velocity (PSV), end-diastolic velocity (EDV) and resistance index (RI).
The signs of ischaemic damage of the anterior and posterior segments of the eye were reduced in rats supplemented with resveratrol during pre-ischaemic period (within 30 days) and post-ischaemic period follow up (30 days). The OBF study in the pre-ischaemic period showed a statistically significant increase in PSV and EDV and decrease in RI in the arterial vessels in rats administered with resveratrol (Group II) as compared to Group I. Analysis of the OBF study results in the post-ischaemic period showed that in 3 days after the retinal ischaemia-reperfusion modeling in Group IA rats there was a statistically significant decrease in PSV, in CRA and LPCA. There was also decrease in OBF velocities in all arteries in Group II, but the average PSV values were significantly higher while the RI in CRA, LPCA was lower (by 16% and 10%, respectively) as compared to Group IA (p<0.05 ). In 7 days after the retinal ischaemia-reperfusion there was the continued decrease in PSV and EDV in CRA and LPCA in both groups. PSV and EDV increased on 30 days after ischaemia in rats supplemented with resveratrol compared to Group IA during post-ischaemic follow up in CRA, LPCA, IOA.
The OBF parameters in the rat model of the retinal ischaemia-reperfusion injury were determined using the ultrasound Doppler methods. There was registered improvement of OBF in rats with ischaemia-reperfusion that were supplemented with resveratrol for a long period (2 months).