First Author: O.Dorokhova UKRAINE
Co Author(s): E. Maltsev O. Zborovska
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To describe in details the heterogeneity of retinal pathological changes in dithizone induced diabetic rabbits.
The Filatov Institute of Eye Diseases and Tissue Therapy, NAMS Ukraine, Odessa, Ukraine.
We modeled diabetes mellitus (DM) in Chinchilla rabbits by dithizone. The observation period was not more then 6 months. Histological sections of enucleated animals’ eyes were stained with hematoxylin-eosin or by McManus-Hotchkiss (PAS-reaction) and then studied by histochemical and histological methods.
We determined destruction of photoreceptor layer, diminishing of neurons quantity in nuclear layers, diminishing of neurons in ganglion cell layer, thinning of plexiform layers, mixing of outer and inner nuclear layers, proliferation of the glial elements, destruction of the pigment epithelium, undulation of retinal inner surface, and retinal atrophic changes in retinas of the diabetic rabbits after 16-17 weeks of observation. All of these described retinal damages were focal and combined with the evident changes in the intensity and the type of PAS- positive substances in its layers. It should be noted that all these destructive and atrophic changes were not uniformly expressed throughout the retina and were focal. Focal changes should be understood as location of the degenerative changes of different severity and depth near the normal retina. Implementation of the morphometric determinations in such model cannot be limited by the measurements of the total thickness, or the thickness of the layers. In such model cluster analysis should be applied for addition to the standard morphometric data.
It should be taking into account when use dithizone induced diabetic rabbits that retinal neurodegenerative changes are focal. Morphometric studies cannot be limited by the measurements of the total thickness, or the thickness of the layers. In such model cluster analysis should be applied for supplementation the standard morphometric data.