First Author: A.Öner TURKEY
Co Author(s): M. Sahiner Z. Gonen D. Bahar M. Unlu
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To evaluate the effects of anti-VEGF drugs on retinal pigment epithelium (RPE) cell cultures.
This is an experimental study
Aflibercept (0.5 mg/ml), bevacizumab (0,3125 mg/ml) and ranibizumab (0.125 mg/ml) were applied to the RPE cell cultures which were isolated from the enucleated eyes of New Zealand white rabbits. Viability, apoptosis, proliferation, and senesence of the cells were evaluated in the control and drug applied cultures at the end of 72 hours.
There was a statistically significant increase in the viability and decrease in the apoptosis of the cells in aflibercept applied culture when compared to the control, bevacizumab and ranibizumab groups (p <0.05). There was a statistically significant increase in apoptosis and decrease in viability in the bevacizumab and ranibizumab-treated groups compared with the control group (p <0.05). There was not a difference between groups when we evaluate the effects on cell proliferation and senescence (p> 0.05).
Our study showed that anti-VEGF drugs had no effects on senescence and proliferation of RPE cells. Aflibercept was found to decrease the apoptosis and increase the viability of the cells. In contrast, ranibizumab and bevacizumab was observed to increase the apoptosis and reduce the viability of RPE cells. In the literature, there are no studies evaluating the effects of anti-VEGF drugs on senesence. We believe that our study will guide future studies in this respect.