First Author: J.Monés SPAIN
Co Author(s): M. Leiva T. Pena G. Martinez M. Biarnes A. Serrano E. Fernandez
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To establish the dose of subretinal sodium iodate (NaIO3) in order to create a toxin-induced large-animal model of selective circumscribed atrophy of outer retinal layers, the retinal pigment epithelium (RPE) and photoreceptors (PR), by spectral domain optical coherence tomography (SD-OCT) and immunocytochemistry.
Institut de la Màcula, Barcelona Barcelona Macula Foundation, Barcelona Research Centre of Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Madrid Departament de Medicina i Cirurgia Animals, Facultat de Veterinaria, Universitat Autònoma de Barcelona, Bellaterra Institute of Bioengineering, Universidad Miguel Hernández, Alicante
Fifteen male and female, healthy Yorkshire pigs received unilateral subretinal escalating doses of NaIO3 under general anaesthesia. In all the animals, volumes of 0.1 to 0.2ml of NaIO3 were injected into the subretinal space of the area centralis, through a 23/38 gauge subretinal cannula. Control SD-OCTs were performed 1 month and 2 months after the surgery, at which time pigs were euthanized and eyes enucleated. Globes were routinely processed for histologic and immunohistochemical evaluation.
SD-OCT and immunohistochemistry revealed circumscribed and well-demarcated funduscopic lesions, limited to the outer retinal layers in pigs treated with 0.01 mg/ml of subretinal sodium iodate.
The generation of a controlled area of circumscribed retinal damage, with well-delimited borders, and selectively of outer layers of the retina presented herein shows several clinical and histological features of geographic atrophy in age-related macular degeneration. This swine model of selective outer retinal damage may represent a valuable tool in the investigation of new emerging regenerative therapies that aim to restore visual function, such as stem cell transplantation or optogenetics.