AMD-Lipidomics: Relevance and interest for screening, follow-up and etiopathogenesis of AMD

Session Details

Session Title: Free Paper Session 22: AMD V

Session Date/Time: Sunday 10/09/2017 | 08:00-09:30

Paper Time: 08:54

Venue: Room 115

First Author: : C.Gonzalez FRANCE

Co Author(s): :                                 

Abstract Details

Purpose:

To evaluate impact of lipidomics study on AMD diagnosis, screening, follow-up, etiopathogenesis, and links, correlations between those 2 entities

Setting:

Interventional, non-comparative, retrospective case series

Methods:

40 patients.10 Normal patients, Group T: control group. 30 AMD patients,7 men,23 women, 3 groups, A,B,C; A:10 patients with first stage AMD, B:10 Atrophy AMD patients (predominant atrophic areas), C:10 patients with Neovascular AMD . Ophthalmologic exam included ETDRS visual acuity (VA), complete ophthalmic examination, Fundus examination, autofluorescence imaging (FAF), (Region Finder Software), optical coherence tomography (HRA Spectral Domain OCT) (OCT en face software, M-S software), and fluorescein angiography (FA) and ICG when Neovascular complication. Lipidomic Study (Metatoul LIPIDOMIQUE, Inserm/Université Paul Sabatier UMR1048): Blood tests and analysis, all lipids qualitative and quantitative analysis, all the same for all patients, whatever group. Blood test is done during ophthalmologic exam. Plasma congelation “snap frost” in liquid nitrogen after total blood centrifugation, then liquid-liquid extraction to enable lipids analysis: neutral lipid by GC, as well as fatty acid but after BF3 methanol derivation, phospholipids by LC-MS directly, whereas sphingolipids are firstly hydrolyzed. Polyinsatured fatty acids (Isoprostan and eicosanoid) metabolites preparation is slightly different: protein precipitation, then pre-concentration by SPE (solid phase extraction) before m’analyses by LC-MS

Results:

Analysis will determine qualitative and quantitative lipids values in each group of patients, and proportion, characterization, singularity of each of them; so, characterization, prevalence, specifics of and for each group. Similar results for group A and C for Total Neutral Lipids, Fame, Free Fatty acid, Oxysterols, Phospholipids. Significative difference for group C with Eicosanoids,Fame free fatty acids; for group B with sphingosins, total neutral lipids, phospholipids total and Phosphatidyl choline; for group A with Oxysterol. Lipidomic study’ evaluation, identification, classification in AMD patients, and especially A, B, C groups allow AMD screening, follow-up, particularly according to AMD type and stage. Lipidomic study may have biomarker feature and let AMD prevention, etiopathogenic concept and therapeutic prospects

Conclusions:

AMD characterization thus through Lipidomic study allow better diagnosis, follow-up, screening of AMD. Interrelations and correlations between AMD and Lipidomics lead to better etiopathogenesis understanding and therapeutics prospects

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