Cytokine profiling in the sub-silicon oil fluid after vitrectomy surgeries for refractory retinal diseases

Session Details

Session Title: Free Paper Session 20: Vitreoretinal Surgery V

Session Date/Time: Sunday 10/09/2017 | 08:00-09:30

Paper Time: 08:24

Venue: Room 117

First Author: : H.Kaneko JAPAN

Co Author(s): :    K. Takayama   T. Tsunekawa   T. Iwase   H. Terasaki                    

Abstract Details

Purpose:

Silicone oil (SO) is an intraocular surgical adjuvant that reduces the surgical complications in refractory retinal diseases, although membrane and cellular proliferation is often seen even in SO-filled eyes. We hypothesized that the fluid in the space between the SO and the retina, named the “sub-silicone oil fluid (SOF),” enhances these biological responses. The aims of this study are to propose a safe method for SOF extraction and to examine the cytokine levels in SOF.

Setting:

In total, 50 SOF samples were collected in this study from which 13 vitreous fluid samples were also collected during the primary vitrectomy surgery who underwent vitrectomy at Nagoya University hospital.

Methods:

We analyzed major inflammatory cytokine expressions and SOF osmotic pressures from eyes with rhegmatogenous retinal detachment (RRD), proliferative diabetic retinopathy (PDR), proliferative vitreoretinopathy (PVR), and macular hole-associated retinal detachment (MHRD).

Results:

Interleukin (IL)-6, monocyte chemotactic protein-1, and vascular endothelial growth factor (VEGF) in the SOF with PDR were significantly higher than in those with RRD or MHRD. Fibroblast growth factor-2, IL-10, IL-12p40, IL-8, VEGF, and transforming growth factor beta 1 levels in eyes with exacerbated PDR indicated a significantly higher expression than those with simple PDR. IL-6 and tumour necrosis factor alpha in eyes with exacerbated PVR demonstrated a significantly higher expression than in those with simple PVR. However, there was no change in SOF osmotic pressure compared to the original vitreous fluid.

Conclusions:

These studies indicate that disease-specific SOF is a significant reflection of disease status.

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