Cytokine profiling in the sub-silicon oil fluid after vitrectomy surgeries for refractory retinal diseases

Session Details

Session Title: Free Paper Session 20: Vitreoretinal Surgery V

Session Date/Time: Sunday 10/09/2017 | 08:00-09:30

Paper Time: 08:24

Venue: Room 117

First Author: : H.Kaneko JAPAN

Co Author(s): :    K. Takayama   T. Tsunekawa   T. Iwase   H. Terasaki                    

Abstract Details


Silicone oil (SO) is an intraocular surgical adjuvant that reduces the surgical complications in refractory retinal diseases, although membrane and cellular proliferation is often seen even in SO-filled eyes. We hypothesized that the fluid in the space between the SO and the retina, named the “sub-silicone oil fluid (SOF),” enhances these biological responses. The aims of this study are to propose a safe method for SOF extraction and to examine the cytokine levels in SOF.


In total, 50 SOF samples were collected in this study from which 13 vitreous fluid samples were also collected during the primary vitrectomy surgery who underwent vitrectomy at Nagoya University hospital.


We analyzed major inflammatory cytokine expressions and SOF osmotic pressures from eyes with rhegmatogenous retinal detachment (RRD), proliferative diabetic retinopathy (PDR), proliferative vitreoretinopathy (PVR), and macular hole-associated retinal detachment (MHRD).


Interleukin (IL)-6, monocyte chemotactic protein-1, and vascular endothelial growth factor (VEGF) in the SOF with PDR were significantly higher than in those with RRD or MHRD. Fibroblast growth factor-2, IL-10, IL-12p40, IL-8, VEGF, and transforming growth factor beta 1 levels in eyes with exacerbated PDR indicated a significantly higher expression than those with simple PDR. IL-6 and tumour necrosis factor alpha in eyes with exacerbated PVR demonstrated a significantly higher expression than in those with simple PVR. However, there was no change in SOF osmotic pressure compared to the original vitreous fluid.


These studies indicate that disease-specific SOF is a significant reflection of disease status.

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