Difference in aqueous humor cytokines among subgroups based on OCT parameters in wet age-related macular degeneration patients

Session Details

Session Title: Free Paper Session 14: AMD IV

Session Date/Time: Friday 08/09/2017 | 16:30-18:00

Paper Time: 16:30

Venue: Room 111

First Author: : S.Moon SOUTH KOREA

Co Author(s): :    H. Kim   J. Joo                          

Abstract Details

Purpose:

To investigate the possible roles of various cytokines and growth factors in the pathogenesis of AMD by comparing aqueous humor levels of 20 cytokines between eyes with cataract and those with AMD. In addition, we also investigated the correlation of morphologic information based on optical coherence tomography (OCT) between cytokine information in AMD eyes.

Setting:

Cross-sectional intervention study

Methods:

The cytokine levels from 13 AMD patients were compared according to the presence of optical coherence tomography (OCT) parameters - such as subretinal fluid (SRF), pigment epithelial detachment (PED), subretinal tissue (SRT), outer retinal tabulation (ORT). The cytokine levels of aqueous humor from 10 cataract patients were analyzed as control. RayBio (RayBiotech, Inc., GA, USA) antibody array were used for measurement of 20 different growth factors and cytokines, such as vascular endothelial growth factor (VEGF), MCP-1, MIP1α, MIP1β, GM-CSF, TNFα, IFN-γ and Interleukins. Differences in the concentrations of growth factors and cytokines in eyes with neovascular AMD compared with control eyes and the influence of presence of OCT based component.

Results:

A significantly increased expression of Monocyte chemoattractant protein-1 (MCP-1) (P=0.001), Macrophage inflammatory protein 1α (MIP-1α) (P=0.021), MIP-1β (P=0.010) and VEGF (P=0.021) were measured in the aqueous humor of eyes with neovascular AMD. AMD patients present with SRF showed significantly low level of proinflammatory cytokines such as Interleukin (IL)-1α and granulocyte monocyte colony stimulating factor (GM-CSF), compared with CNV patients without SRF (P=0.036, P=0.05). CNV patients present with PED showed relatively low level of inflammatory cytokines such as GM-CSF and IFN-γ and TNFα, compared with PED absent group (P=0.006, P=0.034, P=0.011). By contrast, CNV patients with SRT showed relatively high level of IFN-γ, compared with SRT absent patients (P=0.045). Compared with controls, type 1 CNV group showed increased level in MCP-1, MIP1α, and MIP1β, (P=0.001, P=0.021, P=0.012) but did not of VEGF (P=0.083). Meanwhile type 2 CNV group showed increased level in MCP-1 and VEGF (P=0.040 ,P=0.040).

Conclusions:

Inflammatory cytokine such as MIP-1α and MIP-1β seemed to be associated with disease pathomechanism of neovascular AMD as well as angiogenic factor. But the concentration of inflammatory cytokine is vary by type of AMD and the presence or absence of OCT based parameters. CNV patients with SRF and/or PED showed low concentration of proinflammatory cytokines may be associated with benign disease course. And the concentration of VEGF and angiogenic cytokine dose not increased significantly in type 1 CNV implies that inhibition of VEGF alone seems to be not enough treatment in case of type1 CNV. So, treatment decisions for chronic neovascular AMD patients should be based on OCT based bio markers and angiographic classification.

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