Integration of submacular hES-RPE monolayers xenografts in monkeys with a disrupted blood retinal barrier

Session Details

Session Title: Free Paper Session 12: Imaging I

Session Date/Time: Friday 08/09/2017 | 14:30-16:00

Paper Time: 15:36

Venue: Room 111

First Author: : C.Dysli SINGAPORE

Co Author(s): :    R. Fink   S. Wolf   M. Zinkernagel                       

Abstract Details

Purpose:

Fluorescence Lifetime Imaging Ophthalmoscopy (FLIO) is a novel imaging technique in ophthalmology. It allows non-invasive in vivo measurement of autofluorescence lifetimes of natural fluorophores of the retina upon excitation with a laser light. FLIO measurements in different retinal diseases showed that the technique is useful to detect early metabolic changes and can differentiate between different retinal deposits. The aim of this study was to characterize fundus autofluorescence lifetimes in retinal drusen due to age-related macular degeneration (AMD).

Setting:

This study was conducted at the department of ophthalmology at the University Hospital of Bern, Switzerland.

Methods:

Fluorescence lifetime imaging was performed using a Fluorescence Lifetime Imaging Ophthalmoscope (Heidelberg Engineering, Heidelberg, Germany). Autofluorescence was elicited with a 473 nm blue laser light and decay times were measured in a short (498–560 nm) and in a long (560–720 nm) spectral channel. Fluorescence lifetime data was compared with corresponding fundus autofluorescence intensity images, spectral domain optical coherence tomography (OCT) and colour fundus images. Soft drusen and reticular pseudodrusen were analysed as separate subgroups and compared to an age matched control group.

Results:

64 eyes from 64 patients with AMD and retinal drusen (age: mean±SD 78±8.5 years; range 59-94 years) were investigated and compared to 20 age matched healthy controls. Mean retinal autofluorescence lifetime in patients with AMD was significantly prolonged compared to the healthy control eyes (mean±SEM; SSC 486±18ps vs 332±11ps, p>0.0001; LSC: 493±9ps vs 382±17ps, p>0.0001). Areas of drusen featured a broader range of fluorescence lifetime values. However, they did not differ significantly from the surrounding retina. Long lifetimes were identified in areas of atrophy and intraretinal hyperreflective deposits identified by OCT. Areas of short lifetime corresponded to deposits within the photoreceptor outer segment band.

Conclusions:

Mean retinal autofluorescence lifetimes in AMD were significantly prolonged compared to healthy control eyes. Intraretinal deposits caused prolonged lifetimes whereas deposits in the area of the outer photoreceptor segments lead to short fluorescence lifetimes.

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