Negative staining of the vitreous with the use of vital dyes

Session Details

Session Title: Free Paper Session 1: Vitreoretinal Surgery I

Session Date/Time: Thursday 07/09/2017 | 09:00-10:30

Paper Time: 09:42

Venue: Room 111

First Author: : C.Mariotti ITALY

Co Author(s): :    M. Nicolai   V. Pirani   S. Donati   M. Reibaldi                    

Abstract Details


To describe a new chromo-vitrectomy technique in order to facilitate the visualization and non-traumatic removal of the vitreous cortex, epiretinal membrane (ERM), and inner limiting membrane (ILM).


Eye clinic, Polytechnic University of Marche, Ancona, Italy


Three commercially available chromo vitrectomy dyes were used in the present study; TWIN® (AL.CHI.MI.A.S.R.L., Italy), MembraneBlue-Dual® (DORC International, Netherlands), and Doubledyne® (Alfa Intes, Italy). The surgeon performed a partial core vitrectomy and induced a posterior vitreous detachment. The vital dye was then injected into the retro hyaloid space in the balanced salt solution (BSS). The surgeon completed the vitrectomy and gradually aspirated the dye with the probe. Once the vitrectomy was completed, the surgeon performed the peeling of the ERM and ILM without additional injection of the dye over the macula.


The presence of the dye over the macula facilitated the visualization of the vitreous cortex by blocking the red reflex and increased the contrast power of the coaxial light probe during the vitrectomy. This allowed a “negative” colouration of the vitreous because the dye acts by increasing the visibility of the surrounding BSS and not the vitreous itself. Among the three dyes, TWIN® staining solution showed high cohesiveness and limited diffusion during the vitrectomy as the dye remained more confined to the posterior pole and thus resulted in higher intraocular visibility and better retinal membranes staining and removal in comparison to the other dyes.


This study describes a new chromo vitrectomy technique using the single injection of the dye to increase the visualization of the vitreous, posterior hyaloid, epiretinal membrane and inner limiting membrane

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